Reporter

Part:BBa_K235013

Designed by: Kyliah Clarkson   Group: iGEM09_VictoriaBC   (2009-09-03)


[K145303][K235000] (ribokey-mediated pLac-controlled GFP reporter)

This composite part will constitutively produce ribolocked GFP mRNA, and will produce the ribokey needed for transcription only when induced by lactose or an analogue like IPTG. This ought to reduce signal noise due to transient pLac activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 736
    Illegal BsaI.rc site found at 1163


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Categories
Parameters
None